polyclonal adam10 c-terminal antibody (Thermo Fisher)

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Thermo Fisher polyclonal adam10 c-terminal antibody
Antibodies and reagents

Polyclonal Adam10 C Terminal Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+adam10+c-terminal+antibody/pooled+adam10+sirna/pmc11533308-10-0-5
Average 90 stars, based on 1 article reviews

polyclonal adam10 c-terminal antibody - by Bioz Stars, 2026-09

90/100 stars

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1) Product Images from "Regulation of ADAM10 activity through microdomain-dependent intracellular calcium changes"

Article Title: Regulation of ADAM10 activity through microdomain-dependent intracellular calcium changes

Journal: Cell Communication and Signaling : CCS

doi: 10.1186/s12964-024-01891-5

Figure Legend Snippet: Antibodies and reagents

Techniques Used: Concentration Assay, Affinity Purification, Control

Figure Legend Snippet: Buffer and solutions

Techniques Used: Lysis, Protease Inhibitor, ALP Assay, Activity Assay, Saline

Calcium- and ADAM10-dependent cleavage of E-cadherin. A - C : Confluent A549 monolayers were starved overnight and stimulated with 10 µM ionomycin, 1 µM thapsigargin, or 100 µM trifluoperazine (TFP) in the absence or presence of 10 µM GI254023X (GI), an ADAM10 inhibitor for 1 h. DMSO (0.1%) served as a vehicle control. Lysates were collected and subjected to E-cadherin cleavage analyses in A and B ( n = 4). In C, cells were transfected with AP-BTC prior to seeding, and the cleavage of alkaline phosphatase (AP)-conjugated betacellulin (BTC) was analyzed via an alkaline phosphatase (AP) assay ( n = 3). The quantitative data are shown as the means + SDs. Asterisks without lines represent differences compared with the control, and those with lines represent differences between groups (* p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001)

Figure Legend Snippet: Calcium- and ADAM10-dependent cleavage of E-cadherin. A - C : Confluent A549 monolayers were starved overnight and stimulated with 10 µM ionomycin, 1 µM thapsigargin, or 100 µM trifluoperazine (TFP) in the absence or presence of 10 µM GI254023X (GI), an ADAM10 inhibitor for 1 h. DMSO (0.1%) served as a vehicle control. Lysates were collected and subjected to E-cadherin cleavage analyses in A and B ( n = 4). In C, cells were transfected with AP-BTC prior to seeding, and the cleavage of alkaline phosphatase (AP)-conjugated betacellulin (BTC) was analyzed via an alkaline phosphatase (AP) assay ( n = 3). The quantitative data are shown as the means + SDs. Asterisks without lines represent differences compared with the control, and those with lines represent differences between groups (* p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001)

Techniques Used: Control, Transfection

Time-dependence of Ca 2+ -induced ADAM10 activation. A - D : Confluent A549 monolayers were starved overnight and stimulated with 10 µM ionomycin or 100 µM TFP in the presence or absence of 10 µM GI for different durations (1’=1 min). DMSO (0.1%) served as a vehicle control. Lysates were collected and subjected to E-cadherin cleavage analyses in A and C ( n = 3). In B and D, cells were transfected with AP-BTC prior to seeding, and AP-BTC cleavage was analyzed via the AP assay ( n = 3). E, F: A549 cells were grown to 70% confluence on poly-L-lysine coated cover slips, and subjected to calcium imaging. Ionomycin (10 µM), 1 µM thapsigargin and 100 µM trifluoperazine, were automatically injected after 1.5 min of baseline measurement in the presence or absence (Ca 2+ sequestration with EGTA) of Ca 2+ in Ringer’s solution, followed by further recording for 20 min. DMSO (0.1%) served as a baseline measurement and vehicle control. The number of experiments across the number of cells N/n is indicated in the figures. The quantitative data are shown as the means + SDs. Asterisks without lines represent differences compared with the control, and those with lines represent differences between groups (* p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001)

Figure Legend Snippet: Time-dependence of Ca 2+ -induced ADAM10 activation. A - D : Confluent A549 monolayers were starved overnight and stimulated with 10 µM ionomycin or 100 µM TFP in the presence or absence of 10 µM GI for different durations (1’=1 min). DMSO (0.1%) served as a vehicle control. Lysates were collected and subjected to E-cadherin cleavage analyses in A and C ( n = 3). In B and D, cells were transfected with AP-BTC prior to seeding, and AP-BTC cleavage was analyzed via the AP assay ( n = 3). E, F: A549 cells were grown to 70% confluence on poly-L-lysine coated cover slips, and subjected to calcium imaging. Ionomycin (10 µM), 1 µM thapsigargin and 100 µM trifluoperazine, were automatically injected after 1.5 min of baseline measurement in the presence or absence (Ca 2+ sequestration with EGTA) of Ca 2+ in Ringer’s solution, followed by further recording for 20 min. DMSO (0.1%) served as a baseline measurement and vehicle control. The number of experiments across the number of cells N/n is indicated in the figures. The quantitative data are shown as the means + SDs. Asterisks without lines represent differences compared with the control, and those with lines represent differences between groups (* p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001)

Techniques Used: Activation Assay, Control, Transfection, Imaging, Injection

Threshold-dependence of Ca 2+ -induced ADAM10 activation. A - F : Confluent A549 monolayers were starved overnight and stimulated with different concentrations of ionomycin in the presence or absence of 10 µM GI for 30 min ( A , B ) or at different time points ( C - F ). DMSO (0.1%) served as a vehicle control. Lysates were collected and subjected to E-cadherin cleavage analysis in A, C, and E ( n = 3). In B, D, and F, the cells were transfected with AP-BTC prior to seeding, and AP-BTC cleavage was analyzed via the AP assay ( n = 3). Asterisks without lines represent differences compared with the control, and those with lines represent differences between groups (* p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001)

Figure Legend Snippet: Threshold-dependence of Ca 2+ -induced ADAM10 activation. A - F : Confluent A549 monolayers were starved overnight and stimulated with different concentrations of ionomycin in the presence or absence of 10 µM GI for 30 min ( A , B ) or at different time points ( C - F ). DMSO (0.1%) served as a vehicle control. Lysates were collected and subjected to E-cadherin cleavage analysis in A, C, and E ( n = 3). In B, D, and F, the cells were transfected with AP-BTC prior to seeding, and AP-BTC cleavage was analyzed via the AP assay ( n = 3). Asterisks without lines represent differences compared with the control, and those with lines represent differences between groups (* p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001)

Techniques Used: Activation Assay, Control, Transfection

Dependence of ADAM10-mediated E-cadherin cleavage on the origin of the increase in calcium concentration. A - F : Confluent A549 monolayers were starved overnight and stimulated with 10 µM Ion or 100 µM TFP in the presence or absence of 10 µM GI for 1 h. DMSO (0.1%) served as a vehicle control. Experiments were performed in the presence (2 mM CaCl 2 ) or nominal absence of calcium ( A , C , F , G ), with extracellular Ca 2+ chelation by 3 mM EGTA (noncell permeable, added 30 min before stimulation) ( B , E , H ) or intracellular Ca 2+ depletion with 10 µM BAPTA-AM (cell loading 30 min prior to stimulation) ( C , F ). Lysates were collected and subjected to E-cadherin cleavage analyses in A to F ( n = 3). ( n = 4). In G and H, cells were transfected with AP-BTC prior to seeding, and AP-BTC cleavage was analyzed via the AP assay ( n = 3). The quantitative data are shown as the means + SDs. Asterisks without lines represent differences compared with the control, and those with lines represent differences between groups (* p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001)

Figure Legend Snippet: Dependence of ADAM10-mediated E-cadherin cleavage on the origin of the increase in calcium concentration. A - F : Confluent A549 monolayers were starved overnight and stimulated with 10 µM Ion or 100 µM TFP in the presence or absence of 10 µM GI for 1 h. DMSO (0.1%) served as a vehicle control. Experiments were performed in the presence (2 mM CaCl 2 ) or nominal absence of calcium ( A , C , F , G ), with extracellular Ca 2+ chelation by 3 mM EGTA (noncell permeable, added 30 min before stimulation) ( B , E , H ) or intracellular Ca 2+ depletion with 10 µM BAPTA-AM (cell loading 30 min prior to stimulation) ( C , F ). Lysates were collected and subjected to E-cadherin cleavage analyses in A to F ( n = 3). ( n = 4). In G and H, cells were transfected with AP-BTC prior to seeding, and AP-BTC cleavage was analyzed via the AP assay ( n = 3). The quantitative data are shown as the means + SDs. Asterisks without lines represent differences compared with the control, and those with lines represent differences between groups (* p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001)

Techniques Used: Concentration Assay, Control, Transfection

Regulation of cell-associated and soluble ADAM10 activity by Ca 2+ transients. A , B : Confluent A549 monolayers were starved overnight and stimulated with 10 µM ionomycin or 100 µM TFP in the presence or absence of 10 µM GI for 1, 10, 30 and 60 min. Lysates were collected and subjected to E-cadherin cleavage analyses. The ratio of mature to pro-form was quantified as measure of maturation by densitometry ( n = 3). C , D : Confluent A549 cell monolayers were starved overnight and stimulated with 10 µM ionomycin, 1 µM thapsigargin or 100 µM TFP for 1 min–1 h. Subsequently, the cells were subjected to surface staining for ADAM10, and the fluorescence intensity was measured by flow cytometry ( n = 4). E - F . Confluent A549 monolayers were starved overnight and stimulated with 10 µM ionomycin or 100 µM TFP in the presence or absence of 10 µM GI for 1 min–1 h. Supernatants were subjected to FRET-based activity measurements ( n = 3). In A to F, 0.1% DMSO served as vehicle control. The data are shown as the means + SD. The statistical analyses was performed using a one-sample t-test followed by an FDR analysis. Asterisks without lines represent differences compared with the control, and those with lines represent differences between groups (* p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001)

Figure Legend Snippet: Regulation of cell-associated and soluble ADAM10 activity by Ca 2+ transients. A , B : Confluent A549 monolayers were starved overnight and stimulated with 10 µM ionomycin or 100 µM TFP in the presence or absence of 10 µM GI for 1, 10, 30 and 60 min. Lysates were collected and subjected to E-cadherin cleavage analyses. The ratio of mature to pro-form was quantified as measure of maturation by densitometry ( n = 3). C , D : Confluent A549 cell monolayers were starved overnight and stimulated with 10 µM ionomycin, 1 µM thapsigargin or 100 µM TFP for 1 min–1 h. Subsequently, the cells were subjected to surface staining for ADAM10, and the fluorescence intensity was measured by flow cytometry ( n = 4). E - F . Confluent A549 monolayers were starved overnight and stimulated with 10 µM ionomycin or 100 µM TFP in the presence or absence of 10 µM GI for 1 min–1 h. Supernatants were subjected to FRET-based activity measurements ( n = 3). In A to F, 0.1% DMSO served as vehicle control. The data are shown as the means + SD. The statistical analyses was performed using a one-sample t-test followed by an FDR analysis. Asterisks without lines represent differences compared with the control, and those with lines represent differences between groups (* p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001)

Techniques Used: Activity Assay, Staining, Fluorescence, Flow Cytometry, Control

Ophiobolin A-induced ADAM10 activity. A , B : Confluent A549 monolayers were starved overnight and stimulated with 10 µM ophiobolin A in the absence or presence of 10 µM GI. DMSO (0.1%) served as a vehicle control. In A, cells were transfected with AP-BTC prior to seeding, and AP-BTC cleavage was analyzed via the AP assay ( n = 3). In B, lysates were collected and subjected to E-cadherin cleavage analyses ( n = 3). The quantitative data are shown as the means + SDs. Asterisks without lines represent differences compared with the control, and those with lines represent differences between groups (* p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001)

Figure Legend Snippet: Ophiobolin A-induced ADAM10 activity. A , B : Confluent A549 monolayers were starved overnight and stimulated with 10 µM ophiobolin A in the absence or presence of 10 µM GI. DMSO (0.1%) served as a vehicle control. In A, cells were transfected with AP-BTC prior to seeding, and AP-BTC cleavage was analyzed via the AP assay ( n = 3). In B, lysates were collected and subjected to E-cadherin cleavage analyses ( n = 3). The quantitative data are shown as the means + SDs. Asterisks without lines represent differences compared with the control, and those with lines represent differences between groups (* p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001)

Techniques Used: Activity Assay, Control, Transfection

Spatial control of ADAM10 activation by Ca 2+ influx via TRP channels. A - C : HEK WT and stable HEK µOR-TRPC4, HEK-TRPM3α2 and HEK-TRPC5 cells were transfected with AP-BTC and stimulated with 10 µM ionomycin, 60 nM Englerin A (EngA) or 100 µM pregnenolone sulfate (PregS) for 30 min in the presence or absence of 10 µM GI. DMSO (0.1%) served as a vehicle control. AP-BTC cleavage was analyzed via the AP assay ( n = 3). D-H: HEK WT and stable HEK µOR-TRPC4, HEK-TRPM3α2 and HEK-TRPC5 cells were seeded on poly-L-lysine coated cover slips, grown to 70% confluence and subjected to calcium imaging. EngA (60 nM) ( A , C ) or 100 µM PregS ( B ) was automatically injected 1.5 min after the baseline measurement, followed by recording for 20 min. DMSO (0.1%) served as the baseline measurement and the vehicle control. The number of experiments across the number of cells N/n is indicated in the figures. The amplitude ( G ) and area under the curve ( H ) were quantified and plotted. The quantitative data are shown as the means + SDs. Statistical analyses were performed using ANOVA followed by Tukey´s post-hoc test for multiple comparisons between groups in G and H. Asterisks without lines represent differences compared with the control, and those with lines represent differences between groups (* p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001)

Figure Legend Snippet: Spatial control of ADAM10 activation by Ca 2+ influx via TRP channels. A - C : HEK WT and stable HEK µOR-TRPC4, HEK-TRPM3α2 and HEK-TRPC5 cells were transfected with AP-BTC and stimulated with 10 µM ionomycin, 60 nM Englerin A (EngA) or 100 µM pregnenolone sulfate (PregS) for 30 min in the presence or absence of 10 µM GI. DMSO (0.1%) served as a vehicle control. AP-BTC cleavage was analyzed via the AP assay ( n = 3). D-H: HEK WT and stable HEK µOR-TRPC4, HEK-TRPM3α2 and HEK-TRPC5 cells were seeded on poly-L-lysine coated cover slips, grown to 70% confluence and subjected to calcium imaging. EngA (60 nM) ( A , C ) or 100 µM PregS ( B ) was automatically injected 1.5 min after the baseline measurement, followed by recording for 20 min. DMSO (0.1%) served as the baseline measurement and the vehicle control. The number of experiments across the number of cells N/n is indicated in the figures. The amplitude ( G ) and area under the curve ( H ) were quantified and plotted. The quantitative data are shown as the means + SDs. Statistical analyses were performed using ANOVA followed by Tukey´s post-hoc test for multiple comparisons between groups in G and H. Asterisks without lines represent differences compared with the control, and those with lines represent differences between groups (* p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001)

Techniques Used: Control, Activation Assay, Transfection, Imaging, Injection

Concentration Assay:

Article Title: Regulation of ADAM10 activity through microdomain-dependent intracellular calcium changes
Article Snippet: Polyclonal ADAM10 C-terminal Antibody , Invitrogen by Thermo Fisher Scientific (Frankfurt, Germany) , 1 μg/ml.

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Concentration Assay:

Affinity Purification:

Control:

Lysis:

Protease Inhibitor:

ALP Assay:

Activity Assay:

Saline:

Transfection:

Activation Assay:

Imaging:

Injection:

Staining:

Fluorescence:

Flow Cytometry:

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